Faults and solutions in the use of liquid chromatograph
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The operators who often use liquid chromatographs must all know that when using the liquid chromatograph, there may be some failures. Today, Shanghai Relievo answers the common problems and solutions for the use of liquid chromatographs.
Liquid chromatography peak bifurcation how is it?
This is usually caused by contamination of the column or collapse of the column packing. For the first case, the column is first flushed back with pure water and then replaced with methanol. The column is then washed with methanol + isopropanol (4 + 6) (the length of the rinse time is determined by the contamination of the sample). Replace it with methanol and rinse with pure water. Finally, rinse the column with methanol for more than 30 minutes. If the peak after washing is still poor, consider the second case. For the second case, unscrew the stigma and check the column packing for induration or collapse. Remove the indurated part (contaminated filler), fill the new filler, drop a drop of methanol, fill the filler, refill it, press it with a stainless steel rod with the same diameter as the top of the column, fill it with flat, drop the methanol, and then compress it repeatedly. Times until filled. The stigma is rinsed with methanol, wipe the packing on the outer wall of the column, tighten the stigma, and rinse with pure methanol for more than 30 minutes.
Why does the "Wungsten Lamp Low Energy" error sometimes occur during self-tests?
In general, the reason is that there are light-blocking objects in the system, the light path is deviated, and the tungsten lamp power system or the tungsten light bulb is broken. At this time, first check whether there is light in the photometric room; open the lid of the detector light source and check whether the light is on; if the light does not turn on, turn off and replace the new lamp. When changing, pay attention to the model; Lamp fuses, to see if it is loose, oxidized, blown; if it fails, it should be replaced immediately; then turn on the power again; if the above failures still occur, reinstall the software and perform the self-test.
What to do if there is a large pressure fluctuation and the flow rate is unstable?
The reason for this is that the air in the system or the jewel ball and the valve seat of the one-way valve have foreign objects, so that the two cannot be sealed. Observe the amount of mobile phase in the processing work to ensure that the stainless steel filter sinks into the bottom of the accumulator to avoid inhaling air and the mobile phase should be fully degassed. If there is a foreign matter between the check valve and the valve seat, remove the check valve and place it in a beaker containing acetone and wash it with ultrasonics.
What are the reasons for the increase in liquid chromatography column pressure?
There are many reasons for the increase in column pressure. This is generally caused by the presence of anomalous mass-occupying objects in the bed, resulting in increased fluid resistance. For example, buffer salts such as (ammonium acetate, etc.) are deposited in the column, or the sample is contaminated. For the first case, the treatment should be performed with pure water at 40 to 50°C, and the column is washed at a low speed in the forward direction. After the column pressure is gradually decreased, the flow rate is increased accordingly. After the column pressure is greatly reduced, rinse with room temperature pure water. Afterwards, the column was rinsed with pure methanol for 30 minutes. For the second case, the contaminated C18 column was caused by the deposition of the sample, and the column was reverse flushed with pure water. Then, the column was changed to methanol, followed by methanol and isopropanol (4+6). Flush the column (the length of the rinse time depends on the contamination of the sample), rinse with methanol, rinse with pure water, and rinse the column for 30 minutes.