SPAD502 Chlorophyll Meter Analysis of Chlorophyll Content in Alpine Rhododendron
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Using the SPAD502 chlorophyll meter, the SPAD values ​​of the second and third leaves (representative of the main functional leaves) of the new shoot tip of the robust and disease-free plants were measured. Each leaf was taken from 6 different sites for determination, and finally taken. average value. The spectrophotometric method determines the absolute content of chlorophyll, while the SPAD502 chlorophyll meter measures the relative amount of chlorophyll, which can be called the leaf color value. Its working principle is to use two different wavelength light sources to illuminate the surface of the plant leaf, and the SPAD value is obtained by comparing the difference in the optical density of light transmitted through the leaf, which is a dimensionless ratio.
The SPAD502 chlorophyll meter was used to determine the relationship between SPAD value and chlorophyll content in the leaves of Fuling Jinling. The chlorophyll content showed an increasing trend with the increase of SPAD value. The best correlation function between chlorophyll a, chlorophyll b, chlorophyll a+b and SPAD values ​​was a power function model. The closeness of the correlation was chlorophyll a> chlorophyll a + b> chlorophyll b, which may be related to the SPAD chlorophyll analyzer. One of the working light sources is 660nm red, which is close to the maximum absorption peak wavelength of chlorophyll a (663nm), but related to the maximum absorption peak wavelength of chlorophyll b (645nm). Similar results are also found in other plants. .